Analysis of the correlation between climatic variables and Dengue cases in the city of Alagoinhas/BA

The Aedes aegypti mosquito is the main vector of dengue and is a synanthropic insect and due to its anthropophilic nature, it has specific reproductive needs. In addition to that, it also needs tropical regions that provide climate-prone conditions that favor vector development. In this article, we propose the cross-correlation analysis between the climatic variables air temperature, relative humidity, weekly average precipitation and dengue cases in the period from 2017 to early 2021 in the municipality of Alagoinhas, Bahia, Brazil. To do so, we apply the trend-free cross-correlation, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\rho DCCA$$\end{document}ρDCCA, being a generalization of the fluctuation analysis without trend, where we calculate the cross correlation between time series to establish the influence of these variables on the occurrence of dengue disease. The results obtained here were a moderate correlation between relative humidity and the incidence of dengue cases, and a low correlation for relative air temperature and precipitation. However, the predominant factor in the incidence of dengue cases in the city of Alagoinhas is relative humidity and not air temperature and precipitation.

www.nature.com/scientificreports/ virus, which can mutate rapidly and create new strains, making it difficult to target with a single vaccine. Furthermore, there are concerns about vaccine-induced enhancement of disease, which has further complicated the development of a safe and effective vaccine. As a result, prevention strategies such as mosquito control and personal protective measures remain the primary methods for reducing the incidence of dengue fever [16][17][18] . They are transmitted to humans by species of mosquitoes of the genus Aedes, with Aedes aegypti being the main vector 12 .
The presence of Aedes Aegypti in urban centers is directly related to climatic conditions and public sanitation, with climatic variables playing a significant role in the distribution of the mosquito 10 . Currently several phenomena have caused global variations in climatic conditions. One example is projections that indicate an increase between 1.8 and 4 • C 19,20 in temperature. In Brazil temperatures are expected to rise between 1.8 and 4 • C [21][22][23] .
Dengue is the most prevalent urban arbovirus in the Americas, mainly in Brazil 24,25 . It is a febrile illness that has been of great importance in public health in recent years [26][27][28] . Dengue virus (DENV) is an arbovirus transmitted by the bite of the female Aedes aegypti mosquito and has four different serotypes (DENV-1, DENV-2, DENV-3 and DENV-4).
Dengue fever affects people of all ages, but adults and young people have been the most affected by the disease since the introduction of the virus in Alagoinhas. However, as of 2006, some states showed the recirculation of serotype DENV2 after a few years of predominance of serotype DENV3 29,30 . This scenario led to an increase in the number of cases of severe forms and hospitalizations in children, mainly in the Northeast of the country [30][31][32] .
These epidemics were characterized by a pattern of reduced severity for children, who accounted for more than 50% of hospitalized patients in municipalities with the largest population 33 . Even in municipalities with a smaller population, more than 25% of patients hospitalized for dengue were children, which highlights that the whole country has been suffering, in a similar way, these changes in the profile of the disease 34 . Despite the reduction in the severity of the disease, dengue remains a public health problem, especially in municipalities with low population, such as Alagoinhas/BA 35 , where this research was carried out.
In this study, we aimed to analyze the cross-correlation between the climatic variables air temperature, relative air humidity and average weekly precipitation and dengue cases in the municipality of Alagoinhas, Bahia, Brazil.
To carry out this work, combined data from two sources were used. The first from the meteorological station in the city of Alagoinhas, Bahia, maintained by the National Institute of Meteorology and epidemiological data from the FioCruz Dengue Observatory 36 . These raw data are collected at latitude: −12.28 , longitude: −38.55 , altitude: 212 m.
In the datasets obtained, we have separated the columns of data corresponding to dengue cases, air temperature, humidity relative air and weekly average precipitation. We used these data to calculate trendless crosscorrelation coefficients, ρDCCA 37 , for air temperature, relative air humidity and precipitation in relation to confirmed cases of dengue, in order to establish the influence of these variables on the occurrence of the disease. We plotted the graph of the ρDCCA s of the collected variables in order to study the influence of these variables on dengue cases. The scales were determined automatically by the MaiaStatistics software 38 which follows the algorithm defined by Peng 39 and Zebende 37 .
The analysis revealed a moderate correlation between relative humidity and the incidence of dengue cases in the municipality of Alagoinhas between the years 2017 and 2020 and a low correlation for relative air temperature and precipitation.

Materials and methods
Alagoinhas is a Brazilian municipality in the state of Bahia, located in the North Coast and Agreste region of Bahia. Its area is 718.089 square kilometers and its population in 2020 was 153.023 inhabitants, therefore having a population density of 210.05 inhabitants per square kilometer. It is limited to the north by the municipality of Inhambupe, to the south by the municipality of Catu, to the east by the municipality of Araçás, to the west by the municipality of Aramari, to the northeast by the municipality of Entre Rios and to the southwest by the municipality of Teodoro Sampaio. The municipality is crossed by the BR-101, which goes towards the State of Sergipe. This municipality was chosen because it presents a significant increase in dengue cases in the region.
The dataset. The study carried out is a computational ecological type based on time series with design between the years 2017 and 2021. Data on the number of confirmed cases were obtained from InfoDengue 36 , which is an alert system for arboviruses based on hybrid data generated through the integrated analysis of data mined from the social web and climatic and epidemiological data.
The meteorological variables, Temperature, Relative Humidity and Precipitation were obtained by a station located in the municipality that collects this data daily 40 .
Data relating to the epidemiological condition of the municipality are reported weekly while climate variables are available daily. So that the time series have the same seasonality, we calculate the weekly average of these variables. Figure 1 shows these data and reveals that the municipality has a temperature with little fluctuation, sparse rain showers and a large variation in relative humidity.
An important factor is the absence of climate data between 2021 and 2022 due to the coronavirus pandemic, since the weather station is manually operated. For this reason, the research was limited to data from 2017 to early 2021.
How calculate the coefficient. The unbiased cross-correlation coefficient, ρDCCA 37 is used to determine whether two time series y e y ′ present negative, null or positive correlation in scale n, presenting values in the range −1 ρDCCA 1 . We calculated ρ DCCA(n), for an n scale using Eq. (1)  39 for time series y on scale n e F DCCA y ′ (n) is the DFA for the time series y ′ at n scale.
To calculate the value of F 2 DCCA (n) we used Eq. (2) where N corresponds to the number of elements in each time series, n to the size of the scale (box) and f 2 DCCA (n.i) corresponds to the mean value of the products of the residues of each series and each range, given by Eq. (3) (1) www.nature.com/scientificreports/ In Eq. (3) R k is the y series integrated into the k box given by Eq. (4) and R k,i is the value of the linear fit, using the method of least squares, for the y series at point i and R ′ k and the y ′ series integrated into box k, given by Eq. (5), while R ′ k,i is the value of the linear fit, by least squares, for the series y ′ , at point i.
To calculate the F DFA (n) of each series in each scale (box 0) n we use Eq. (6), which corresponds to the value of the square root of the squared residues, in each box, where y(k) is the value of the series in the point k and y n (k) is the value of the linear fit, using the method of least squares, for the y-series, at point k.
We used Eqs.

Results
The city of Alagoinhas is located close to the equator ( 12 • 8 ′ 9 ′′ S 38 • 25 ′ 8 ′′ W), which makes the seasons difficult to be characterized, presenting a climate between 2017 and 2021 with low annual precipitation. The remarkable interannual variability of rainfall, associated with low total annual rainfall values is a reflection of the macroclimate of the Northeast region of Brazil and one of the main factors for the occurrence of "drought" events, characterized by a sharp reduction in total seasonal rainfall during the rainy period.
The interannual variability of rainfall in the city is associated with variations in Sea Surface Temperature (SST) patterns over the tropical oceans, which affect the position and intensity of the Intertropical Convergence Zone (ITCZ) over the Atlantic Ocean 42 .
The city of Alagoinhas has a measured rainfall described in Fig. 2, which demonstrates that rainfall comes seasonally, abruptly and in short periods of time. Temperatures, as shown in Fig. 1, shows a little variation, with a maximum recorded of 35 • C and a minimum of 20 • C, which can occur during all seasons of the year, with greater variations in the hottest months of the year, January and March, and the mildest between June and September.
The average relative humidity is 48%, with a maximum of 76% and a minimum of 20%. These data indicate stability in the humidity index in the months of July to September while in the others there are both high or low peaks.
The period of study of dengue cases in the municipality was 4 years, in which 1.483 confirmed cases were registered, which indicates an incidence in the population, in the described period, of about 35%, according to the methodology described in the technical note on dengue from the Council National Health Secretariats on Dengue Incidence Rate.
Observing Fig. 1, we do not perceive a direct relationship of growth and decrease between the time series of registered cases of dengue and the climatic variables. Thus, in this study we seek a deeper understanding of the cross-relationship between these series using the cross-correlation coefficient ρDCCA. Figures 3 and 4 shows this result, which shows an average correlation between relative humidity and confirmed cases of dengue and a low correlation between the variables relative air temperature and precipitation. Although several authors 43 claim that the variables air temperature and precipitation have a direct influence on the incidence of dengue, in the city of Alagoinhas these variables are a little less relevant than humidity.
According to the six levels described in Table 1 (three positive and three negative) in this article, we can associate the color with a range of ρDCCA. In our case, the ρDCCA value was positive for the humidity being www.nature.com/scientificreports/ in the yellow range. This perception is not observed for the other variables that remained in the range between blue and light green. For Alagoinhas, and the data obtained (shown in Table 2), we observed a localized level of anti-correlation (blue) and n ≈ 2 . In the windows n ≈ 32 , an average correlation for humidity is clearly perceived while weak for temperature and precipitation.

Conclusion
In this article, we seek to understand the correlation between the time series related to relative humidity, air temperature, precipitation and confirmed cases of dengue in the municipality of Alagoinhas/BA from 2017 to 2021. We compared weekly data and observed the existence of an inverse correlation between air temperature and precipitation and a direct correlation between humidity and dengue cases. We apply the detrended crosscorrelation coefficiente, ρDCCA , to measure this two-by-two correlation of these non-stationary series. The interesting fact observed is that, different from what is stated in the literature, the predominant factor in the incidence of dengue cases in the city of Alagoinhas is the relative humidity of the air and not the air temperature and precipitation.

Data availability
The data and materials used in this research are available upon request. Interested parties may contact me at mbfigueredo@uneb.br to obtain a copy of the data and materials. Data and materials will be made available in compliance with ethical and legal requirements, and any restrictions on access to the data will be disclosed to interested parties. The datasets generated and/oor analysed during the current study are available in the "INFO DENGUE" repository https:// info. dengue. mat. br/ and "Agritempo" https:// www. agrit empo. gov. br/ agrit empo/ jsp/ Pesqu isaCl ima/ index. jsp? sigla UF= BA.